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Testicular Tissue Freezing
At Mamata Fertility Hospital in Secunderabad, we are dedicated to providing advanced fertility preservation solutions to help individuals and families plan for the future. One such innovative option is Testicular Tissue Freezing, a groundbreaking method designed to safeguard male fertility. This procedure offers hope and flexibility for individuals facing medical challenges or planning to preserve their reproductive abilities for later in life. With our state-of-the-art facilities and a compassionate team of experts, we are here to guide you every step of the way.
QUICK FACTS
- Hospital stay: Approx 1 day
- Anaesthesia: Local
- Covered by health insurance? Yes
- Procedure duration: ~20 minutes.

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What is Testicular Tissue Freezing?
Testicular tissue freezing is a cutting-edge fertility preservation technique where a small sample of testicular tissue is extracted, frozen, and stored for future use. This tissue contains spermatogenic cells, which can mature to produce sperm required for conception.
This method is particularly beneficial for individuals who cannot produce viable sperm through ejaculation or other conventional means. Unlike sperm freezing, which requires mature sperm, testicular tissue freezing focuses on preserving the very foundation of sperm production — the cells within the testicular tissue.
Future advancements in medical science may enable the tissue to be reimplanted into the body or used in laboratory settings to create sperm for fertility treatments like IVF (in vitro fertilization) or ICSI (intracytoplasmic sperm injection).
Benefits of Testicular Tissue Freezing
Testicular tissue freezing provides essential benefits for those wishing to preserve their fertility under varying circumstances. At Mamata Fertility Hospital, we recommend this procedure for a variety of reasons:
1. Preserves Future Fertility
This procedure offers long-term fertility preservation, allowing individuals to plan for parenthood when the time is right.
2. Ideal for Prepubescent Boys
It is the only fertility preservation method available for young boys who have yet to produce mature sperm, for example, those undergoing medical treatments.
3. Supports Patients Facing Cancer Treatments
Men and boys scheduled to undergo treatments like chemotherapy or radiation, which could damage reproductive abilities, can freeze their testicular tissue in advance.
4. Bolsters Research and New Treatment Options
By preserving immatures spermatogenic cells, this technique paves the way for future scientific advancements in the field of reproductive medicine.
5. Offers Psychological Reassurance
Knowing that your reproductive potential is preserved can provide emotional comfort and reduce stress during challenging life circumstances.
Who Can Benefit from Testicular Tissue Freezing?
Testicular tissue freezing can be life-changing for individuals across various situations. Here’s a closer look at who might benefit from this remarkable option:
1. Prepubescent Boys Facing Cancer Treatments
Boys who haven’t yet reached puberty and are undergoing cancer treatments like chemotherapy or radiation can preserve their testicular tissue for safeguarding future fertility.
2. Men with Non-Obstructive Azoospermia
Adult men with a condition where no sperm is present in their semen due to impaired sperm production can consider this option.
3. Cancer Patients
Men and boys diagnosed with cancers, especially those requiring gonadotoxic treatments (treatment that may harm reproductive cells), should explore testicular tissue freezing.
4. Men with Genetic Disorders
Men with conditions like Klinefelter syndrome or other genetic disorders that put them at risk of infertility may find testicular tissue freezing invaluable.
5. Those Planning to Delay Parenthood
Men who wish to delay fatherhood but are at risk of fertility decline due to age, medical conditions, or work-related hazards can benefit from freezing their testicular tissue.
6. Men Preparing for Pelvic or Testicular Surgery
Men undergoing procedures likely to affect their reproductive capabilities can preserve their tissue as a precaution.
The Testicular Tissue Freezing Procedure
At Mamata Fertility Hospital, we prioritize patient safety, comfort, and effective outcomes. The procedure for testicular tissue freezing is thoughtfully designed and carried out by skilled specialists. Here’s how it works:
1. Initial Consultation and Screening
Our team of fertility experts conducts a personalized consultation. We review your medical history, assess fertility goals, and determine the suitability of the procedure.
2. Surgical Retrieval of Tissue
- A minor surgical procedure is performed under general or local anesthesia.
- A small piece of testicular tissue is removed through a minimally invasive approach, ensuring minimal discomfort and a quick recovery.
3. Laboratory Preparation
- The retrieved tissue is processed in our cutting-edge laboratory under controlled conditions.
- Spermatogenic cells are identified and prepared for freezing to maintain their viability.
4. Cryopreservation and Long-Term Storage
- The tissue is frozen using vitrification, a rapid freezing technique that prevents ice crystal formation.
- Samples are then securely stored in cryopreservation tanks at extremely low temperatures (-196°C) for indefinite preservation.
Success Rates
While testicular tissue freezing is still advancing, it offers promising success rates depending on individual factors, such as the quality of the retrieved tissue and the individual’s age at the time of freezing. Pioneering success stories globally highlight its potential.
Factors Influencing Success:
- Age – Younger ages at the time of tissue retrieval often yield better-quality spermatogenic cells.
- Underlying Medical Condition – The likelihood of successful use depends on the reason for preservation, such as cancer or genetic conditions.
- Future Technological Advancements – Emerging techniques may enhance the usability of frozen tissue in fertility treatments.
At Mamata Fertility Hospital, our specialists stay at the forefront of research developments to ensure we provide the best possible care and outcomes for our patients.
Cost of Testicular Tissue Freezing in Secunderabad
We are committed to providing affordable and accessible fertility preservation services. Here’s a cost breakdown for testicular tissue freezing in Secunderabad at Mamata Fertility Hospital:
- Consultation and Initial Assessment: ₹10,000 – ₹15,000
- Surgical Retrieval Procedure: ₹60,000 – ₹90,000
- Cryopreservation and First-Year Storage: ₹25,000 – ₹40,000
- Annual Storage Fees: ₹10,000 – ₹15,000
Our financial counselors are happy to assist with transparent pricing details and flexible payment options to help you plan for your fertility care with confidence.
Making an Informed Decision
Deciding on testicular tissue freezing is a significant moment in your fertility preservation journey. At Mamata Fertility Hospital, we are here to empower you with the knowledge and support needed to move forward confidently.
Steps to Making a Thoughtful Choice:
- Consult Our Experts – Discuss your individual circumstances, medical history, and fertility goals with our specialists.
- Understand the Benefits – Evaluate how this procedure could fit into your long-term plans.
- Plan Ahead – Consider the financial, medical, and emotional aspects of testicular tissue freezing as part of your future family planning goals.
- Feel Empowered – With our compassionate care and advanced technology, you can take proactive steps toward preserving your reproductive health.
FAQs
1. What is the difference between sperm freezing and testicular tissue freezing?
Sperm freezing preserves mature sperm, while testicular tissue freezing stores tissue containing immature spermatogenic cells essential for sperm development.
2. How long can testicular tissue remain frozen?
The tissue can be stored indefinitely with no loss in viability when frozen under proper conditions.
3. Can tissue reimplantation restore fertility?
Reimplantation of frozen tissue holds promise for restoring fertility and hormonal function, especially for those who cannot produce sperm naturally.
4. Is the procedure safe for children?
Yes, testicular tissue freezing is a safe procedure for prepubescent boys, offering them a chance to preserve their future fertility.
5. Does the surgical procedure have risks?
The procedure is minimally invasive and carries low risk, with most patients recovering quickly.
6. Is testicular tissue freezing considered experimental?
While the process is recognized and widely practiced, its applications in creating sperm from preserved tissue are still being researched.
7. Is the frozen tissue usable in current fertility treatments?
Yes, preserved tissue may be used in certain cases today, and ongoing developments in reproductive science are broadening its applications.
8. Who should consider testicular tissue freezing?
It is ideal for prepubescent boys, cancer patients, and men with conditions preventing natural sperm production.
We Can Help You With All The Possible Infertility Treatments.
WHAT PATIENTS EXPERIENCE AT MAMATA FERTILITY?
“I had an excellent first visit to Mamata Hospitals, where I met Dr. Mamata madam and Dr. Arti. Madam and Dr cheatn The process was systematic, instilling confidence that my issues will be effectively addressed. The staff and doctors were incredibly friendly and supportive, making me feel at ease. Their professionalism and warmth have given me great confidence in their care. I look forward to sharing more about my journey in detail.”
Vishu S.N
At Mamata Fertility Hospital in Secunderabad, we are committed to helping you make informed decisions and providing world-class fertility care. Explore how testicular tissue freezing can offer hope and flexibility for your future.
Contact us today to book a consultation and take control of your reproductive possibilities!

Dr Aarti Deenadayal Tolani
MBBS, MS ( OBGYN), FICOG
Clinical Director, Scientific In- Charge & Fertility Consultant with 15+ years Of Experience